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Human PDPN protein

SKU: orb392264

Description

Recombinant human PDPN protein is produced by our Mammalian expression system and the target gene encoding Ala23-Thr131 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceASTGQPEDDT ETTGLEGGVA MPGAEDDVVT PGTSEDRYKS GLTTLVATSV NSVTGIRIED LPTSESTVHA QEQSPSATAS NVATSHSTEK VDGDTQTTVE KDGLSTVTLV DHHHHHH
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
DisclaimerFor research use only

Alternative Names

Podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha; T1A; PDPN; GP36

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human PDPN protein (orb392264)

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Available Sizes

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10 μg
$ 200.00
50 μg
$ 490.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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