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Catalog Number | orb392259 |
---|---|
Category | Proteins |
Description | Recombinant human PDIA3 protein is produced by our mammalian expression system and the target gene encoding Ser25-Leu505 is expressed with a 6His tag at the C-terminus. |
Target | PDIA3 |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | SDVLELTDDN FESRISDTGS AGLMLVEFFA PWCGHCKRLA PEYEAAATRL KGIVPLAKVD CTANTNTCNK YGVSGYPTLK IFRDGEEAGA YDGPRTADGI VSHLKKQAGP ASVPLRTEEE FKKFISDKDA SIVGFFDDSF SEAHSEFLKA ASNLRDNYRF AHTNVESLVN EYDDNGEGII LFRPSHLTNK FEDKTVAYTE QKMTSGKIKK FIQENIFGIC PHMTEDNKDL IQGKDLLIAY YDVDYEKNAK GSNYWRNRVM MVAKKFLDAG HKLNFAVASR KTFSHELSDF GLESTAGEIP VVAIRTAKGE KFVMQEEFSR DGKALERFLQ DYFDGNLKRY LKSEPIPESN DGPVKVVVAE NFDEIVNNEN KDVLIEFYAP WCGHCKNLEP KYKELGEKLS KDPNIVIAKM DATANDVPSP YEVRGFPTIY FSPANKKLNP KKYEGGRELS DFISYLQREA TNPPVIQEEK PKKKKKAQED LVDHHHHHH |
UniProt ID | P30101 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Alternative names | ERP57; ERP60; GRP58; Protein disulfide-isomerase A Read more... |
Note | For research use only |
Entrez | 2923 |
ICC, IF, IHC, IP, WB | |
Bovine, Canine, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Greater than 85% as determined by SDS-PAGE. | |
59.3 kDa | |
E.coli |
Human | |
1.25 ng/mL-80 ng/mL | |
0.31 ng/mL |
IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |