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Catalog Number | orb707022 |
---|---|
Category | Proteins |
Description | Recombinant Human PDGFR alpha Protein is produced by mammalian expression system and the target gene encoding Gln24-Glu524 is expressed with a 6His at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | PDGFRA |
Entrez | 5156 |
UniProt ID | P16234 |
Protein Sequence | QLSLPSILPN ENEKVVQLNS SFSLRCFGES EVSWQYPMSE EESSDVEIRN EENNSGLFVT VLEVSSASAA HTGLYTCYYN HTQTEENELE GRHIYIYVPD PDVAFVPLGM TDYLVIVEDD DSAIIPCRTT DPETPVTLHN SEGVVPASYD SRQGFNGTFT VGPYICEATV KGKKFQTIPF NVYALKATSE LDLEMEALKT VYKSGETIVV TCAVFNNEVV DLQWTYPGEV KGKGITMLEE IKVPSIKLVY TLTVPEATVK DSGDYECAAR QATREVKEMK KVTISVHEKG FIEIKPTFSQ LEAVNLHEVK HFVVEVRAYP PPRISWLKNN LTLIENLTEI TTDVEKIQEI RYRSKLKLIR AKEEDSGHYT IVAQNEDAVK SYTFELLTQV PSSILDLVDD HHGSTGGQTV RCTAEGTPLP DIEWMICKDI KKCNNETSWT ILANNVSNII TEIHSRDRST VEGRVTFAKV EETIAVRCLA KNLLGAENRE LKLVAPTLRS EHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | PDGFR2 protein, RHEPDGFRA protein, Platelet-derive Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
PLA, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.4 kDa after removal of the signal peptide. The apparent molecular mass of PDGFRA-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |