Human PDGF BB Protein

• Catalog Number: orb429455
• Category: Proteins
• Description: Recombinant of human,yeast PDGF B protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The PDGF-BB was lyophilized from 0.2 µm filtered solution in 20mM sodium phosphate in absence of any carrier protein.
• Purity: Greater than 98.0% as determined by SDS-PAGE.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu
• Application notes: Cytokines And Growth Factors
• Source: Saccharomyces cerevisiae
• Biological Activity: The ED50, as measured by its ability to stimulate proliferation of mouse 3T3 fibroblasts is typically 1 ng/ml, corresponding to a Specific Activity of 1,000,000IU/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
• Alternative names: Glioma-derived growth factor, GDGF, Osteosarcoma-d
• Note: For research use only