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Catalog Number | orb429455 |
---|---|
Category | Proteins |
Description | Recombinant of human,yeast PDGF B protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 98.0% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu |
Source | Saccharomyces cerevisiae |
Biological Activity | The ED50, as measured by its ability to stimulate proliferation of mouse 3T3 fibroblasts is typically 1 ng/ml, corresponding to a Specific Activity of 1,000,000IU/mg. |
Storage | Stability: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Buffer/Preservatives | The PDGF-BB was lyophilized from 0.2 µm filtered solution in 20mM sodium phosphate in absence of any carrier protein. |
Alternative names | Glioma-derived growth factor, GDGF, Osteosarcoma-d Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
15.1 kDa | |
Unconjugated Human PDGF-BB, His, (orb334919) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1). |
Greater than 98% as determined by reducing SDS-PAGE. | |
12.42 KDa | |
Mammalian |
Greater than 95.0% as determined by SDS-PAGE. | |
Escherichia Coli |
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |