Human PDGF-BB Protein, His-Avi Tag

SKU: orb334919

Description

Unconjugated Human PDGF-BB, His, is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag . The protein has a calculated MW of 15.1 kDa. The protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE)

Key Properties

• Expression System: E. coli
• Target: PDGF-BB
• Reactivity: Human
• Tag: C-6×His & C-Avi
• Molecular Weight: 15.1 kDa
• Expression Region: Ser 82 - Thr 190
• Purity: 95%
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: 0.085% TFA in 30% ACN
• Disclaimer: For research use only

SDS-PAGE analysis of Human PDGF-B protein

Line graph illustrates about the sensitivity range of Human PDGF-B protein using different concentrations

Immobilized Unconjugated Human PDGF-BB, His, Avitag (Cat. No. orb334919) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. orb1146967) with a linear range of 0.5-16 ng/mL (QC tested).

Quick Database Links

Gene Symbol

PDGF-BB

UniProt

P01127