Human PD1 protein

SKU: orb392246

Description

Recombinant human PD1 protein is produced by our Mammalian expression system and the target gene encoding Leu25-Gln167 is expressed with a mFc tag at the C-terminus.

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Reactivity: Human
• Protein Sequence: LDSPDRPWNP PTFSPALLVV TEGDNATFTC SFSNTSESFV LNWYRMSPSN QTDKLAAFPE DRSQPGQDCR FRVTQLPNGR DFHMSVVRAR RNDSGTYLCG AISLAPKAQI KESLRAELRV TERRAEVPTA HPSPSPRPAG QFQPRDCGCK PCICTVPEVS SVFIFPPKPK DVLTITLTPK VTCVVVDISK DDPEVQFSWF VDDVEVHTAQ TQPREEQFNS TFRSVSELPI MHQDWLNGKE FKCRVNSAAF PAPIEKTISK TKGRPKAPQV YTIPPPKEQM AKDKVSLTCM ITDFFPEDIT VEWQWNGQPA ENYKNTQPIM DTDGSYFVYS KLNVQKSNWE AGNTFTCSVL HEGLHNHHTE KSLSHSPGK
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PD1; Programmed cell death protein 1; Protein PD-1; hPD-1; CD279

Immobilized Anti-PDCD1 antibody at 2 μg/ml (100 μl/well) can bind Recombinant Human PDCD1 Protein. The ED50 of Recombinant Human PDCD1 Protein is 0.012 μg/ml.

Quick Database Links

UniProt

Q15116

NCBI Gene ID

5133