Human PD-1 / PDCD1 Protein

• Catalog Number: orb257756
• Category: Proteins
• Description: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
• Reactivity: Human
• Tag: C-Twin-Strep
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 19.0 kDa
• Target: PD-1
• Protein Sequence: NP 005009.2
• Source: Human PD-1, Strep Tag (orb257756) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
• Expression System: HEK293
• Expression Region: Leu 25 - Gln 167
• Endotoxins: 1.0 EU per μg
• NCBI: 005009.2
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: PDCD1,PD1,CD279,SLEB2
• Note: For research use only
• Application notes: This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 19.0 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PD-1 protein

Line graph illustrates about the sensitivity range of Human PD-1 protein using different concentrations

Line graph illustrates about the sensitivity range of Human PD-1 protein using different concentrations