Human PCSK9 Protein, His Tag

• Catalog Number: orb257745
• Category: Proteins
• Description: Human PCSK9 (D374Y), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 13.8 kDa and 58.2 kDa
• Target: PCSK9
• UniProt ID: Q8NBP7
• Protein Sequence: AAI66619.1 (D374Y)
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Gln 31 - Gln 692
• Endotoxins: 1.0 EU per μg
• NCBI: D374, AAI66619.1
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively. The protein migrates as 15 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations