Human PCSK9 Protein, His Tag

SKU: orb257745

Description

Human PCSK9 (D374Y), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively. The protein migrates as 15 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR.

Key Properties

• Expression System: HEK293
• Target: PCSK9
• Reactivity: Human
• Tag: C-6×His
• Molecular Weight: 13.8 kDa and 58.2 kDa
• Expression Region: Gln 31 - Gln 692
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations

Quick Database Links

Gene Symbol

PCSK9

UniProt

Q8NBP7

RefSeq:Q8NBP7, D374