Human PARP1 protein

SKU: orb420172

Description

Human PARP1 protein

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  PARP1 (N-term ZF1) protein has been tested in SDS-Protein and western blot and is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting.

Key Properties

• Expression System: E. coli
• Purity: PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity ≥90%.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

SDS-PAGE results of PARP1 (N-term ZF1) Control Protein. Lane 1: reduced PARP1 N-Term ZF1 protein. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced PARP1 N-Term ZF1 protein. Load: 1 µg. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.

Western Blot of recombinant PARP1 with rabbit anti-PARP1 (N-term ZF1) antibody. Lane 1: PARP1-Zinc Finger domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (N-term ZF1) antibody at 1 µg/ml for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 13 kDa for rPARP1 (N-term ZF1). Other band(s): none.

Quick Database Links

UniProt

P09874