Human PARP1 protein

SKU: orb420172

Description

Human PARP1 protein

Research Area

Cell Biology

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  PARP1 (N-term ZF1) protein has been tested in SDS-Protein and western blot and is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody . Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting

Key Properties

• Expression System: E. coli
• Target: PARP1
• Conjugation: Unconjugated
• Purity: PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity ≥90%.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL

Quick Database Links

Gene Symbol

PARP1

UniProt

P09874