Human PARP1 protein

• Catalog Number: orb420172
• Category: Proteins
• Description: Human PARP1 protein
• Tested applications: SDS-PAGE, WB
• Concentration: 1.0 mg/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity ≥90%.
• Conjugation: Unconjugated
• UniProt ID: P09874
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransfe
• Note: For research use only
• Application notes: PARP1 (N-term ZF1) protein has been tested in SDS-Protein and western blot and is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting.
• Expiration Date: 6 months from date of receipt.