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Catalog Number | orb49648 |
---|---|
Category | Proteins |
Description | Recombinant Human Serine Protease Inhibitor-clade E1 is produced by our Mammalian expression system and the target gene encoding Val24-Pro402 is expressed with a 6His tag at the C-terminus. |
Tag | C-His |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0. |
UniProt ID | P05121 |
Expression System | Human Cells |
Biological Origin | Human |
Expression Region | Val24-Pro402 |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Alternative names | Plasminogen Activator Inhibitor 1; PAI; PAI-1; End Read more... |
Note | For research use only |
Application notes | 6His tag at the C-terminus. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
46.7 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.6 kDa after removal of the signal peptide. The apparent molecular mass of PAI1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
98.00% | |
50.1 kDa (predicted) |