Human Osteopontin protein

• Catalog Number: orb392516
• Category: Proteins
• Description: Recombinant human Osteopontin protein is produced by our Mammalian expression system and the target gene encoding Ile17-Asn314 is expressed with a 6His tag at the C-terminus.
• Target: SPP1
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Buffer/Preservatives: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVNVD HHHHHH
• UniProt ID: P10451
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Source: Human cells
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Alternative names: BNSP; OPN; Osteopontin; Bone sialoprotein 1; Nephr
• Note: For research use only
• Entrez: 6696