Human Osteopontin Protein, His Tag

Catalog Number: orb257724

Catalog Number	orb257724
Category	Proteins
Description	Human Osteopontin, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016).
Target	Osteopontin
Conjugation	Unconjugated
Tag	C-6×His
Reactivity	Human
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Purity	90%
Protein Sequence	AAH07016
UniProt ID	P10451
MW	33.0 kDa
Application notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 12 kDa, 18 kDa and 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression System	HEK293
Endotoxins	0.1 EU per μg
Biological Origin	Human
Expression Region	Ile 17 - Asn 300
Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Note	For research use only
NCBI	AAH07016

SDS-PAGE analysis of Human Osteopontin protein

Human Osteopontin Protein, His Tag [orb2696812]
Purity:
> 95% as determined by SDS-PAGE
Sizes Available: 500 μg, 100 μg

Purity:	> 87% by SDS-PAGE.
Source:	Recombinant Human Osteopontin Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_001035147.1) fused with a 6��His Tag at the C-terminus.

Sizes Available: 50 μg, 100 μg

Purity:	91.60%
MW:	35 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation)

Purity:	98.00%
MW:	34.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Purity:	95.30%
MW:	The recombinant human SPP1 consists of 185 amino acids and predicts a molecular mass of 20.64 kDa.