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| Catalog Number | orb257724 |
|---|---|
| Category | Proteins |
| Description | Human Osteopontin, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
| Target | Osteopontin |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| UniProt ID | AAH07016 |
| MW | 33.0 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 12 kDa, 18 kDa and 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Expression Region | Ile 17 - Asn 300 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

SDS-PAGE analysis of Human Osteopontin protein
>90% as determined by SDS-PAGE | |
34.8 kDa |
> 87% by SDS-PAGE. | |
Recombinant Human Osteopontin Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_001035147.1) fused with a 6��His Tag at the C-terminus. |
>90% as determined by SDS-PAGE. | |
18.36 kDa |
>90% as determined by SDS-PAGE. | |
36.02 kDa |
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