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Catalog Number | orb427498 |
---|---|
Category | Tools |
Description | Recombinant of human Osteocrin protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
Protein Sequence | MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG |
Source | Escherichia Coli |
Storage | Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C |
Buffer/Preservatives | Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer, pH 4.0. |
Alternative names | Osteocrin, Musclin, OSTN. Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 12 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
11.6 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human OSTN(Val28-Gly133) was fused with the N-terminal His Tag and expressed in E. coli. |
98.00% | |
90-100 KDa (reducing condition) |
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