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Catalog Number | orb427476 |
---|---|
Category | Tools |
Description | Recombinant of human OPG protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK |
Source | Escherichia Coli |
Biological Activity | The activity is determined by its ability to neutralize the stimulation of U937 cells treated with 10ng/ml of soluble RANKL corresponding to a specific activity of 100,000IU/mg. |
Storage | Stability: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The OPG was lyophilized from a 0.2µm filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4. |
Alternative names | TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitor Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 12 months from date of receipt. |
Unconjugated | |
95% | |
44.4 kDa | |
Human Osteoprotegerin, His Tag (orb257888) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537). |
Unconjugated | |
92% | |
70.2 kDa | |
Human Osteoprotegerin, Fc Tag (orb257890) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537). |
Greater than 95% as determined by SDS-PAGE. | |
44.65 kDa | |
Mammalian cell |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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