Human NPPB protein

SKU: orb392199

Description

Recombinant human NPPB protein is produced by our E. coli expression system and the target gene encoding His27-His134 is expressed with a 6His tag at the N-terminus.

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, 20% Glycerol, pH 7.4
• Disclaimer: For research use only

Alternative Names

Natriuretic peptides B; Gamma-brain natriuretic peptide

Quick Database Links

UniProt

P16860

NCBI Gene ID

4879