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Catalog Number | orb392197 |
---|---|
Category | Proteins |
Description | Recombinant human NOL3 protein is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed with a GST tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | NOL3 |
Entrez | 8996 |
UniProt ID | O60936 |
Protein Sequence | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGNA QERPSETIDR ERKRLVETLQ ADSGLLLDAL LARGVLTGPE YEALDALPDA ERRVRRLLLL VQGKGEAACQ ELLRCAQRTA GAPDPAWDWQ HVGPGYRDRS YDPPCPGHWT PEAPGSGTTC PGLPRASDPD EAGGPEGSEA VQSGTPEEPE PELEAEASKE AEPEPEPEPE LEPEAEAEPE PELEPEPDPE PEPDFEERDE SEDS |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0. |
Alternative names | Myp; NOL3; Nop30; Nucleolar protein 3; apoptosis r Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
38.6 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human NOL3(Met1-Ser208) was fused without Tag and expressed in E. coli. |
≥90% as determined by SDS-PAGE | |
This protein contains the human NOL3(Met1-Ser208) was fused with the N-terminal Gst Tag and expressed in E. coli. |
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