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Catalog Number | orb392195 |
---|---|
Category | Proteins |
Description | Recombinant human NME1 protein is produced by our E.coli expression system and the target gene encoding Met1-Glu152 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | NME1 |
Entrez | 4830 |
UniProt ID | P15531 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 10% Glycerol, pH 7.5. |
Alternative names | NDPKA; NM23; Nucleoside diphosphate kinase A; NDK Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
18.75 pg/mL-1200 pg/mL | |
4.68 pg/mL |
Unconjugated | |
98% | |
18.0 kDa | |
Human NME1, His Tag (orb257714) is expressed from E.coli cells. It contains AA Ala 2 - Glu 152 (Accession # AAH00293). |
Greater than 90% as determined by SDS-PAGE. | |
44 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
44 kDa | |
E.coli |
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