Human NEDD8 protein

• Catalog Number: orb392178
• Category: Proteins
• Description: Recombinant human NEDD8 protein is produced by our E. coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MGHHHHHHGS LQDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM DSLRFLYDGI RIQADQAPED LDMEDNDIIE AHREQIGGML IKVKTLTGKE IEIDIEPTDK VERIKERVEE KEGIPPQQQR LIYSGKQMND EKTAADYKIL GGSVLHLVLA LRGG
• UniProt ID: Q15843
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Source: E. coli
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Alternative names: NEDD8; Neddylin; Neural precursor cell expressed d
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 4738
• Expiration Date: 6 months from date of receipt.