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Catalog Number | orb392165 |
---|---|
Category | Proteins |
Description | Recombinant human NADK protein is produced by our E. coli expression system and the target gene encoding Ser64-Gly446 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | NADK |
Entrez | 65220 |
UniProt ID | O95544 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MSLHGPCPVT TFGPKACVLQ NPQTIMHIQD PASQRLTWNK SPKSVLVIKK MRDASLLQPF KELCTHLMEE NMIVYVEKKV LEDPAIASDE SFGAVKKKFC TFREDYDDIS NQIDFIICLG GDGTLLYASS LFQGSVPPVM AFHLGSLGFL TPFSFENFQS QVTQVIEGNA AVVLRSRLKV RVVKELRGKK TAVHNGLGEK GSQAAGLDMD VGKQAMQYQV LNEVVIDRGP SSYLSNVDVY LDGHLITTVQ GDGVIVSTPT GSTAYAAAAG ASMIHPNVPA IMITPICPHS LSFRPIVVPA GVELKIMLSP EARNTAWVSF DGRKRQEIRH GDSISITTSC YPLPSICVRD PVSDWFESLA QCLHWNVRKK QAHFEEEEEE EEEG |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 7.5. |
Alternative names | NAD kinase; Poly(P/ATP NAD kinase Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human NADK(Ser64-Gly446) was fused with the N-terminal His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
25.7 kDa | |
E.Coli |
ELISA, WB | |
Greater than 85% by SDS-PAGE gel analyses | |
42.6 kDa | |
E.Coli |
98.00% | |
49 KDa, reducing conditions |
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