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Product Overview
Product Name Human MMP-2 (HEK) protein
Catalog Number orb427159
ReactivityHuman
ConjugationUnconjugated
Target MMP-2 (HEK)
Product Properties
Form/Appearance The MMP-2 is supplied as a sterile Filtered colorless solution.
The MMP-2 is supplied as a 0.2μm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Note For research use only.
Purity >95% as determined by SDS-PAGE.
Source HEK293 cells.
Activity The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/μg.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP2 to 100μg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 1 hour.
Product Description

Recombinant of human MMP-2 (HEK) protein

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