Human MMP-2 (HEK) Protein

SKU: orb427159

Description

Recombinant of human MMP-2 (HEK) protein

Images & Validation

• Application Notes:
  Enzymes

Key Properties

• Source: HEK293 cells
• Biological Activity: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND)., The specific activity is > 1,000 pmoles/min/µg.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma,) and 100mM stock in DMSO.3. Incubate at 37°C for 1 hour.
• Purity: Greater than 95% as determined by SDS-PAGE.

Storage & Handling

• Storage: Stability: Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles
• Form/Appearance: The MMP-2 is supplied as a sterile Filtered colorless solution.
• Buffer/Preservatives: The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II.