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Catalog Number | orb429445 |
---|---|
Category | Proteins |
Description | Recombinant of human MIP3 beta protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS |
Source | Escherichia Coli |
Biological Activity | The Activity of CCL19 is calculated by the ability to chemoattract Human T cells using a concentration of 7-40ng/ml corresponding to a Specific Activity of 25,000-143,000IU/mg. |
Storage | Stability: Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized without any additives. |
Alternative names | Small inducible cytokine A19, CCL19, Macrophage in Read more... |
Note | For research use only |
Application notes | Chemokines |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of CCL19-hFc is approximately 25-55 kDa due to glycosylation. | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
35.8 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. | |
Mammalian |
Human | |
7.82-500pg/mL | |
4.69pg/mL |