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| Catalog Number | orb427124 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human MIP 3a protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Protein Sequence | ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM |
| Application notes | Chemokines |
| Source | Escherichia Coli |
| Biological Activity | Determined by its ability to chemoattract human T lymphocytes using a concentration range of 10.0 -50.0 ng/ml, corresponding to a specific activity of 20,000-100,000units/mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized MIP-3 Alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized MIP-3a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL20 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | S Small inducible cytokine A20 precursor, CCL20, M Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.3 kDa after removal of the signal peptide.The apparent molecular mass of CCL20-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
>95% as determined by SDS-PAGE | |
14 kDa |
>90% as determined by SDS-PAGE | |
26.1 kDa |
Human | |
15.6 pg/mL-1000 pg/mL | |
3.9 pg/mL |
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