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Catalog Number | orb427094 |
---|---|
Category | Proteins |
Description | Recombinant of human MICA (His) protein |
Form/Appearance | Sterile Filtered clear solution. |
Buffer/Preservatives | MICA protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | MEPHSLRYNL TVLSWDGSVQ SGFLTEVHLD GQPFLRCDRQ KCRAKPQGQW AEDVLGNKTW DRETRDLTGN GKDLRMTLAH IKDQKEGLHS LQEIRVCEIH EDNSTRSSQH FYYDGELFLS QNLETEEWTM PQSSRAQTLA MNVRNFLKED AMKTKTHYHA MHADCLQELR RYLKSGVVLR RTVPPMVNVT RSEASEGNIT VTCRASGFYP WNITLSWRQD GVSLSHDTQQ WGDVLPDGNG TYQTWVATRI CQGEEQRFTC YMEHSGNHST HPVPSLEHHH HHH |
Application notes | Recombinant & Natural Proteins |
Source | Escherichia Coli |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | MHC class I polypeptide-related sequence A, MIC-A, Read more... |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 12.5 kDa after removal of the signal peptide. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of MICA-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |