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Catalog Number | orb392137 |
---|---|
Category | Proteins |
Description | Recombinant human MIA protein is produced by our E. coli expression system and the target gene encoding Gly25-Gln131 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | MIA |
Entrez | 8190 |
UniProt ID | Q16674 |
Protein Sequence | MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQLE HHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Melanoma-derived growth regulatory protein; Melano Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
12.1 kDa | |
E.Coli |
Human | |
7.82-500pg/mL | |
4.69 pg/mL |
Human | |
0.3-90ng/mL | |
0.149ng/mL |
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