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Human METAP1D protein

SKU: orb392133

Description

Recombinant human METAP1D protein is produced by our E. coli expression system and the target gene encoding Arg44-Ala335 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMGSSHHHHHH SSGLVPRGSH MRQRDISHSI VLPAAVSSAH PVPKHIKKPD YVTTGIVPDW GDSIEVKNED QIQGLHQACQ LARHVLLLAG KSLKVDMTTE EIDALVHREI ISHNAYPSPL GYGGFPKSVC TSVNNVLCHG IPDSRPLQDG DIINIDVTVY YNGYHGDTSE TFLVGNVDEC GKKLVEVARR CRDEAIAACR AGAPFSVIGN TISHITHQNG FQVCPHFVGH GIGSYFHGHP EIWHHANDSD LPMEEGMAFT IEPIITEGSP EFKVLEDAWT VVSLDNQRSA QFEHTVLITS RGAQILTKLP HEALEHHHHH H
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris HCl, pH 8.0.
DisclaimerFor research use only

Alternative Names

MAP1D; Methionine aminopeptidase 1D mitochondrial; MAP 1D; MetAP 1D; Methionyl aminopeptidase type 1D mitochondrial; Peptidase M 1D

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human METAP1D protein (orb392133)

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Available Sizes

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10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 3,100.00
DispatchUsually dispatched within 5-10 working days
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