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Catalog Number | orb392133 |
---|---|
Category | Proteins |
Description | Recombinant human METAP1D protein is produced by our E. coli expression system and the target gene encoding Arg44-Ala335 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris HCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | METAP1D |
Entrez | 254042 |
UniProt ID | Q6UB28 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MRQRDISHSI VLPAAVSSAH PVPKHIKKPD YVTTGIVPDW GDSIEVKNED QIQGLHQACQ LARHVLLLAG KSLKVDMTTE EIDALVHREI ISHNAYPSPL GYGGFPKSVC TSVNNVLCHG IPDSRPLQDG DIINIDVTVY YNGYHGDTSE TFLVGNVDEC GKKLVEVARR CRDEAIAACR AGAPFSVIGN TISHITHQNG FQVCPHFVGH GIGSYFHGHP EIWHHANDSD LPMEEGMAFT IEPIITEGSP EFKVLEDAWT VVSLDNQRSA QFEHTVLITS RGAQILTKLP HEALEHHHHH H |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris HCl, pH 8.0. |
Alternative names | MAP1D; Methionine aminopeptidase 1D mitochondrial; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human METAP1D(Arg44-Ala335) was fused with the N/C-terminal His Tag and expressed in E. coli. |
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