Human MEK1 Protein

• Catalog Number: orb427063
• Category: Proteins
• Description: Recombinant of human MEK1 protein
• Form/Appearance: Sterile Filtered clear solution.
• Buffer/Preservatives: MEK1 is supplied at a concentration of in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Application notes: Protein Kinases
• Source: Sf9 Insect Cells
• Biological Activity: ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
• Storage: Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles
• Note: For research use only