Human MEK1 Protein

SKU: orb427063

Description

Recombinant of human MEK1 protein

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  Protein Kinases

Key Properties

• Source: Sf9 Insect Cells
• Biological Activity: ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
• Purity: Greater than 90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles
• Form/Appearance: Sterile Filtered clear solution.
• Buffer/Preservatives: MEK1 is supplied at a concentration of in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only