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Catalog Number | orb427050 |
---|---|
Category | Proteins |
Description | Recombinant of human MDH1 protein |
Form/Appearance | Sterile Filtered colorless solution. |
Buffer/Preservatives | The MDH1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, and 10% glycerol. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH |
Application notes | Enzymes |
Source | Escherichia Coli |
Biological Activity | Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Alternative names | MDH-s, MDHA, MOR2. |
Note | For research use only |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
> 90% as determined by SDS-PAGE. | |
63.13 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
36.6 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human MDH1(Ser2-Ala334) was fused with the C-terminal His Tag and expressed in E. coli. |