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Human MAP1LC3A protein

SKU: orb392119

Description

Recombinant human MAP1LC3A protein is produced by our E. coli expression system and the target gene encoding Met1-Phe121 is expressed with a 6His tag at the C-terminus.

Research Area

Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG FLEHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris, 20% Glycerol, 0.1M NaCl, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human MAP1LC3A protein (orb392119)

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Available Sizes

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10 μg
$ 260.00
50 μg
$ 600.00
500 μg
$ 3,450.00
DispatchUsually dispatched within 5-10 working days
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