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Catalog Number | orb392114 |
---|---|
Category | Proteins |
Description | Recombinant human MAG protein is produced by our Mammalian expression system and the target gene encoding Gly20-Pro516 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | MAG |
Entrez | 4099 |
UniProt ID | P20916 |
Protein Sequence | GHWGAWMPSS ISAFEGTCVS IPCRFDFPDE LRPAVVHGVW YFNSPYPKNY PPVVFKSRTQ VVHESFQGRS RLLGDLGLRN CTLLLSNVSP ELGGKYYFRG DLGGYNQYTF SEHSVLDIVN TPNIVVPPEV VAGTEVEVSC MVPDNCPELR PELSWLGHEG LGEPAVLGRL REDEGTWVQV SLLHFVPTRE ANGHRLGCQA SFPNTTLQFE GYASMDVKYP PVIVEMNSSV EAIEGSHVSL LCGADSNPPP LLTWMRDGTV LREAVAESLL LELEEVTPAE DGVYACLAEN AYGQDNRTVG LSVMYAPWKP TVNGTMVAVE GETVSILCST QSNPDPILTI FKEKQILSTV IYESELQLEL PAVSPEDDGE YWCVAENQYG QRATAFNLSV EFAPVLLLES HCAAARDTVQ CLCVVKSNPE PSVAFELPSR NVTVNESERE FVYSERSGLV LTSILTLRGQ AQAPPRVICT ARNLYGAKSL ELPFQGAHRL MWAKIGPVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
Alternative names | GMA; Myelin-associated glycoprotein; Siglec-4a Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
56.7 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
70.7 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.5 kDa after removal of the signal peptide. The apparent molecular mass of MAG-His is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
81.1 kDa | |
Human MAG, Fc Tag (orb612111) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Pro 516 (Accession # P20916-1). |
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