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Catalog Number | orb392114 |
---|---|
Category | Proteins |
Description | Recombinant human MAG protein is produced by our Mammalian expression system and the target gene encoding Gly20-Pro516 is expressed with a 6His tag at the C-terminus. |
Target | MAG |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | GHWGAWMPSS ISAFEGTCVS IPCRFDFPDE LRPAVVHGVW YFNSPYPKNY PPVVFKSRTQ VVHESFQGRS RLLGDLGLRN CTLLLSNVSP ELGGKYYFRG DLGGYNQYTF SEHSVLDIVN TPNIVVPPEV VAGTEVEVSC MVPDNCPELR PELSWLGHEG LGEPAVLGRL REDEGTWVQV SLLHFVPTRE ANGHRLGCQA SFPNTTLQFE GYASMDVKYP PVIVEMNSSV EAIEGSHVSL LCGADSNPPP LLTWMRDGTV LREAVAESLL LELEEVTPAE DGVYACLAEN AYGQDNRTVG LSVMYAPWKP TVNGTMVAVE GETVSILCST QSNPDPILTI FKEKQILSTV IYESELQLEL PAVSPEDDGE YWCVAENQYG QRATAFNLSV EFAPVLLLES HCAAARDTVQ CLCVVKSNPE PSVAFELPSR NVTVNESERE FVYSERSGLV LTSILTLRGQ AQAPPRVICT ARNLYGAKSL ELPFQGAHRL MWAKIGPVDH HHHHH |
UniProt ID | P20916 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | GMA; Myelin-associated glycoprotein; Siglec-4a |
Note | For research use only |
Entrez | 4099 |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
70.7 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.5 kDa after removal of the signal peptide. The apparent molecular mass of MAG-His is approximately 70-130 kDa due to glycosylation. | |
Mammalian |