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Catalog Number | orb392090 |
---|---|
Category | Proteins |
Description | Recombinant human LRG1 protein is produced by our mammalian expression system and the target gene encoding Val36-Gln347 is expressed with a Fc, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris, 200mM NaCl, 10% Glycerol, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | LRG1 |
Entrez | 116844 |
UniProt ID | P02750 |
Protein Sequence | VTLSPKDCQV FRSDHGSSIS CQPPAEIPGY LPADTVHLAV EFFNLTHLPA NLLQGASKLQ ELHLSSNGLE SLSPEFLRPV PQLRVLDLTR NALTGLPPGL FQASATLDTL VLKENQLEVL EVSWLHGLKA LGHLDLSGNR LRKLPPGLLA NFTLLRTLDL GENQLETLPP DLLRGPLQLE RLHLEGNKLQ VLGKDLLLPQ PDLRYLFLNG NKLARVAAGA FQGLRQLDML DLSNNSLASV PEGLWASLGQ PNWDMRDGFD ISGNPWICDQ NLSDLYRWLQ AQKDKMFSQN DTRCAGPEAV KGQTLLAVAK SQVDDIEGRM DEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris, 200mM NaCl, 10% Glycerol, pH 8.0. |
Alternative names | LRG; Leucine-rich alpha-2-glycoprotein; LRG Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 35.2 kDa after removal of the signal peptide. The apparent molecular mass of LRG1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
≥90% as determined by SDS-PAGE | |
This protein contains the human LRG1(Val36-Gln347) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
≥90% as determined by SDS-PAGE | |
This protein contains the human LRG1(Val36-Gln347) was fused with the C-terminal Fc Tag and expressed in Mammalian cells. |
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