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Human LIPF protein

SKU: orb392085

Description

Recombinant human LIPF protein is produced by our Mammalian expression system and the target gene encoding Leu20-Lys398 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceLFGKLHPGSP EVTMNISQMI TYWGYPNEEY EVVTEDGYIL EVNRIPYGKK NSGNTGQRPV VFLQHGLLAS ATNWISNLPN NSLAFILADA GYDVWLGNSR GNTWARRNLY YSPDSVEFWA FSFDEMAKYD LPATIDFIVK KAGQKQLHYV GHSQGTTIGF IAFSTNPSLA KRIKTFYALA PVATVKYTKS LINKLRFVPQ SLFKFIFGDK IFYPHNFFDQ FLATEVCSRE MLNLLCSNAL FIICGFDSKN FNTSRLDVYL SHNPAGTSVQ NMFHWTQAVK SGKFQAYDWG SPVQNRMHYD QSQPPYYNVT AMNVPIAVWN GGKDLLADPQ DVGLLLPKLP NLIYHKEIPF YNHLDFIWAM DAPQEVYNDI VSMISEDKKV DHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 25mM Tris-HCl, 100mM glycine, 10% Glycerol, pH 7.3.
DisclaimerFor research use only

Alternative Names

Gastric triacylglycerol lipase; GL; Gastric lipase

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human LIPF protein (orb392085)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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