Human LIF Protein

• Catalog Number: orb257990
• Category: Proteins
• Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 19.9 kDa
• Target: LIF
• Source: Human LIF Protein, premium grade (orb257990) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human LIF Protein, premium grade at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag with a linear range of 4-63 ng/mL (QC tested).
• Expression Region: Ser 23 - Phe 202
• Endotoxins: 0.1 EU per μg
• NCBI: AAH69540.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: LIF,CDF,DIA,HILDA,MLPLI
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human LIF protein

Immobilized Human LIF Protein, premium grade (Cat. No. orb257990) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 4-63 ng/mL (QC tested).