Human LIF Protein

• Catalog Number: orb257990
• Category: Proteins
• Description: Human LIF Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1).
• Target: LIF
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• Protein Sequence: AAH69540.1
• UniProt ID: P15018
• MW: 19.9 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.1 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human LIF Protein, premium grade at 0.2 µg/mL (100 µL/well) can bind Human LIF R, Fc Tag with a linear range of 4-63 ng/mL (QC tested).
• Expression Region: Ser 23 - Phe 202
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• NCBI: AAH69540.1
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human LIF protein

Immobilized Human LIF Protein, premium grade (Cat. No. orb257990) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 4-63 ng/mL (QC tested).