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| Catalog Number | orb392070 |
|---|---|
| Category | Proteins |
| Description | Recombinant human LGALS1 protein is produced by our E. coli expression system and the target gene encoding Ala2-Asp135 is expressed with a 6His tag at the C-terminus. |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 10mM PB, 200mM NaCl, 2mM DTT, pH 7.0. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFDLEHHHH HH |
| UniProt ID | P09382 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | E. coli |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | Galectin-1; Gal-1; 14 kDa laminin-binding protein; Read more... |
| Research Area | Apoptotic, Cancer, Epigenetics, Neuroscience, Sign Read more... |
| Note | For research use only |
| Entrez | 3956 |
Human | |
0.16-10ng/mL | |
0.10 ng/mL |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 15.78 KDa. Observed: 15 KDa, reducing conditions |
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