Human Leptin tA Protein

SKU: orb426845

Description

Recombinant of human Leptin tA protein

Images & Validation

• Application Notes:
  Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)

Key Properties

• Source: Escherichia coli
• Biological Activity: Capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake. Its in vivo activity compared to that of PEG-MLA is 9-27 fold higher.
• Purity: Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.

Storage & Handling

• Storage: Stability: Lyophilized PEG-SHLA although stable at room temperature for several weeks, should be stored desiccated below - 2°C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml of PEG-SHLA and filter sterilization mLEP mutant can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles
• Form/Appearance: White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Disclaimer: For research use only