Human Leptin Protein

SKU: orb426828

Description

Recombinant of human Leptin protein

Research Area

Metabolism Research

Images & Validation

• Application Notes:
  Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard

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Key Properties

• Source: Escherichia Coli
• Biological Activity: Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
• Purity: Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln

Storage & Handling

• Storage: Stability: Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OB Protein, Obesity Protein, OBS, Obesity factor.