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Catalog Number | orb392068 |
---|---|
Category | Proteins |
Description | Recombinant human LDLR protein is produced by our mammalian expression system and the target gene encoding Ala22-Arg788 is expressed with a AVI, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | LDLR |
Entrez | 3949 |
UniProt ID | P01130 |
Protein Sequence | AVGDRCERNE FQCQDGKCIS YKWVCDGSAE CQDGSDESQE TCLSVTCKSG DFSCGGRVNR CIPQFWRCDG QVDCDNGSDE QGCPPKTCSQ DEFRCHDGKC ISRQFVCDSD RDCLDGSDEA SCPVLTCGPA SFQCNSSTCI PQLWACDNDP DCEDGSDEWP QRCRGLYVFQ GDSSPCSAFE FHCLSGECIH SSWRCDGGPD CKDKSDEENC AVATCRPDEF QCSDGNCIHG SRQCDREYDC KDMSDEVGCV NVTLCEGPNK FKCHSGECIT LDKVCNMARD CRDWSDEPIK ECGTNECLDN NGGCSHVCND LKIGYECLCP DGFQLVAQRR CEDIDECQDP DTCSQLCVNL EGGYKCQCEE GFQLDPHTKA CKAVGSIAYL FFTNRHEVRK MTLDRSEYTS LIPNLRNVVA LDTEVASNRI YWSDLSQRMI CSTQLDRAHG VSSYDTVISR DIQAPDGLAV DWIHSNIYWT DSVLGTVSVA DTKGVKRKTL FRENGSKPRA IVVDPVHGFM YWTDWGTPAK IKKGGLNGVD IYSLVTENIQ WPNGITLDLL SGRLYWVDSK LHSISSIDVN GGNRKTILED EKRLAHPFSL AVFEDKVFWT DIINEAIFSA NRLTGSDVNL LAENLLSPED MVLFHNLTQP RGVNWCERTT LSNGGCQYLC LPAPQINPHS PKFTCACPDG MLLARDMRSC LTEAEAAVAT QETSTVRLKV SSTAVRTQHT TTRPVPDTSR LPGATPGLTT VEIVTMSHQA LGDVAGRGNE KKPSSVRGLN DIFEAQKIEW HELENLYFQG HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4. |
Alternative names | Low-density lipoprotein receptor; LDL receptor Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
85.9 kDa | |
Human LDL R, His Tag (orb257663) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Arg 788 (Accession # NP_000518.1). |
Unconjugated | |
90% | |
13.8 kDa and 58.2 kDa | |
Human PCSK9 (D374Y), His Tag (orb257745) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)). |
Unconjugated | |
90% | |
13.8 kDa and 59.2 kDa | |
Human PCSK9, His Tag (orb257737) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Human | |
31.25 pg/mL-2000 pg/mL | |
7.8 pg/mL |
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