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| Catalog Number | orb392060 |
|---|---|
| Category | Proteins |
| Description | Recombinant human LCAT protein is produced by our mammalian expression system and the target gene encoding Phe25-Glu440 is expressed with a 6His tag at the C-terminus. |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM Acetate Buffer pH 4.0. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | FWLLNVLFPP HTTPKAELSN HTRPVILVPG CLGNQLEAKL DKPDVVNWMC YRKTEDFFTI WLDLNMFLPL GVDCWIDNTR VVYNRSSGLV SNAPGVQIRV PGFGKTYSVE YLDSSKLAGY LHTLVQNLVN NGYVRDETVR AAPYDWRLEP GQQEEYYRKL AGLVEEMHAA YGKPVFLIGH SLGCLHLLYF LLRQPQAWKD RFIDGFISLG APWGGSIKPM LVLASGDNQG IPIMSSIKLK EEQRITTTSP WMFPSRMAWP EDHVFISTPS FNYTGRDFQR FFADLHFEEG WYMWLQSRDL LAGLPAPGVE VYCLYGVGLP TPRTYIYDHG FPYTDPVGVL YEDGDDTVAT RSTELCGLWQ GRQPQPVHLL PLHGIQHLNM VFSNLTLEHI NAILLGAYRQ GPPASPTASP EPPPPEVDHH HHHH |
| UniProt ID | P04180 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | Phosphatidylcholine-sterol acyltransferase; Lecith Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 3931 |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.47-30 ng/mL | |
0.19 ng/mL |
Human | |
1.57-100ng/mL | |
0.94 ng/mL |
Greater than 90% as determined by SDS-PAGE. | |
50.3 kDa | |
E.coli |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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