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Catalog Number | orb392060 |
---|---|
Category | Proteins |
Description | Recombinant human LCAT protein is produced by our mammalian expression system and the target gene encoding Phe25-Glu440 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM Acetate Buffer pH 4.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | LCAT |
Entrez | 3931 |
UniProt ID | P04180 |
Protein Sequence | FWLLNVLFPP HTTPKAELSN HTRPVILVPG CLGNQLEAKL DKPDVVNWMC YRKTEDFFTI WLDLNMFLPL GVDCWIDNTR VVYNRSSGLV SNAPGVQIRV PGFGKTYSVE YLDSSKLAGY LHTLVQNLVN NGYVRDETVR AAPYDWRLEP GQQEEYYRKL AGLVEEMHAA YGKPVFLIGH SLGCLHLLYF LLRQPQAWKD RFIDGFISLG APWGGSIKPM LVLASGDNQG IPIMSSIKLK EEQRITTTSP WMFPSRMAWP EDHVFISTPS FNYTGRDFQR FFADLHFEEG WYMWLQSRDL LAGLPAPGVE VYCLYGVGLP TPRTYIYDHG FPYTDPVGVL YEDGDDTVAT RSTELCGLWQ GRQPQPVHLL PLHGIQHLNM VFSNLTLEHI NAILLGAYRQ GPPASPTASP EPPPPEVDHH HHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM Acetate Buffer pH 4.0. |
Alternative names | Phosphatidylcholine-sterol acyltransferase; Lecith Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
29.8 kDa | |
Human Apo-A1, His Tag (orb257190) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gln 267 (Accession # NP_000030.1). |
Greater than 90% as determined by SDS-PAGE. | |
50.3 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human LCAT(Phe25-Glu440) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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