Human Laminin521 E8 Protein (Liquid)

• Catalog Number: orb3002501
• Category: Proteins
• Description: Recombinant Human Laminin521 is produced by our Mammalian expression system and the target gene encoding Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a His, Flag and HA tag at N terminus respectively.
• Tag: N-His&N-Flag&N-HA
• Form/Appearance: Supplied as a 0.2 μm filtered solution of 20mM PB，2% Sucrose，4% Mannitol，0.02%Tween-80，pH 7.4.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• UniProt ID: O15230, P11047, P55268
• MW: Predicted: 86.7&25.8&28.5 kDa. Observed: (28-32)&(38-48)&(100-120) kDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: ＜0.01EU/μg as determined by LAL test.
• Biological Activity: ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His)(Cat#orb2992902) at 5μg/ml (100 μl/well) can bind Recombinant Human Laminin521 E8 Protein (Liquid)(Cat#orb3002501)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human Laminin521 E8 Protein (Liquid)(Cat#orb3002501) is 16.99ng/ml.(Regularly tested)
• Expression Region: Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609
• Storage: Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
• Alternative names: Laminin 521; LN521
• Note: For research use only