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| Catalog Number | orb3002505 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Laminin121 E8 is produced by our Mammalian expression system and the target gene encoding Phe1878-Gln2700&Leu1573–Gln1798&Asn1364–Pro1609 is expressed with a 6His, Flag and HA tag at the N terminus respectively. |
| Tag | N-6His &N-Flag&N-HA |
| Form/Appearance | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.(QC Verified). |
| UniProt ID | P11047, P25391, P55268 |
| MW | Predicted: 91.4&25.8&28.5kDa. Observed: (30-33)&(36-48)&(95-140)kDa, reducing conditions |
| Expression System | Human cells |
| Endotoxins | <0.01EU/μg as determined by LAL test. |
| Biological Activity | ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 1 Heterodimer (C-Flag, C-6His)(Cat#orb2992511) at 1μg/ml (100 μl/well) can bind Recombinant Human Laminin121 E8 Protein(Cat#orb3002505)*.The ED50 of Recombinant Human Laminin 121 E8 Protein(Cat#orb3002505) is 82.62ng/ml. *: Biotinylated by NHS-biotin prior to testing.(Regularly tested). |
| Expression Region | Phe1878-Gln2700&Leu1573–Gln1798&Asn1364–Pro1609 |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Alternative names | Laminin 121; LN121 |
| Note | For research use only |
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