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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | KNG1 |
| Reactivity | Human |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | QESQSEEIDC NDKDLFKAVD AALKKYNSQN QSNNQFVLYR ITEATKTVGS DTFYSFKYEI KEGDCPVQSG KTWQDCEYKD AAKAATGECT ATVGKRSSTK FSVATQTCQI TPAEGPVVTA QYDCLGCVHP ISTQSPDLEP ILRHGIQYFN NNTQHSSLFM LNEVKRAQRQ VVAGLNFRMT YSIVQTNCSK ENFLFLTPDC KSLWNGDTGE CTDNAYIDIQ LRIASFSQNC DIYPGKDFVQ PPTKICVGCP RDIPTNSPEL EETLTHTITK LNAENNATFY FKIDNVKKAR VQVVAGKKYF IDFVARETTC SKESNEELTE SCETKKLGQS LDCNAEVYVV PWEKKIYPTV NCQPLGMISL MKRPPGFSPF RSSRIGEIKE ETTSHLRSCE YKGRPPKAGA EPASEREVSL DHHHHHH |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μM filtered solution of 20mM Hac-NaAC, 150mM NaCl, pH 4.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BDK; KNG; Kininogen-1; Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK; Williams-Fitzgerald-Flaujeac factor
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis






