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Catalog Number | orb392025 |
---|---|
Category | Proteins |
Description | Recombinant human KHK protein is produced by our mammalian expression system and the target gene encoding Met1-Val298 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 50nM KCl, 10% Glycerol, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | KHK |
Entrez | 3795 |
UniProt ID | P50053 |
Protein Sequence | MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIVVD HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 50nM KCl, 10% Glycerol, pH 7.4. |
Alternative names | Ketohexokinase; Hepatic fructokinase Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
59.7 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human KHK(Met1-Val298) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
Greater than 90% as determined by SDS-PAGE. | |
59.7 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
59.7 kDa | |
E.coli |
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