Human KARS protein

• Catalog Number: orb392022
• Category: Proteins
• Description: Recombinant human KARS protein is produced by our mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: KARS
• Entrez: 3735
• UniProt ID: Q15046
• Protein Sequence: AAVQAAEVKV DGSEPKLSKN ELKRRLKAEK KVAEKEAKQK ELSEKQLSQA TAAATNHTTD NGVGPEEESV DPNQYYKIRS QAIHQLKVNG EDPYPHKFHV DISLTDFIQK YSHLQPGDHL TDITLKVAGR IHAKRASGGK LIFYDLRGEG VKLQVMANSR NYKSEEEFIH INNKLRRGDI IGVQGNPGKT KKGELSIIPY EITLLSPCLH MLPHLHFGLK DKETRYRQRY LDLILNDFVR QKFIIRSKII TYIRSFLDEL GFLEIETPMM NIIPGGAVAK PFITYHNELD MNLYMRIAPE LYHKMLVVGG IDRVYEIGRQ FRNEGIDLTH NPEFTTCEFY MAYADYHDLM EITEKMVSGM VKHITGSYKV TYHPDGPEGQ AYDVDFTPPF RRINMVEELE KALGMKLPET NLFETEETRK ILDDICVAKA VECPPPRTTA RLLDKLVGEF LEVTCINPTF ICDHPQIMSP LAKWHRSKEG LTERFELFVM KKEICNAYTE LNDPMRQRQL FEEQAKAKAA GDDEAMFIDE NFCTALEYGL PPTAGWGMGI DRVAMFLTDS NNIKEVLLFP AMKPEDKKEN VATTDTLEST TVGTSVVDHH HHHH
• Source: Human cells
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.
• Alternative names: KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA syntheta
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.