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Catalog Number | orb391210 |
---|---|
Category | Proteins |
Description | Recombinant human Interferon gamma protein is produced by our E. coli expression system and the target gene encoding Ser2-Pro147 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | AIF1 |
Entrez | 199 |
UniProt ID | P55008 |
Protein Sequence | SQTRDLQGGK AFGLLKAQQE ERLDEINKQF LDDPKYSSDE DLPSKLEGFK EKYMEFDLNG NGDIDIMSLK RMLEKLGVPK THLELKKLIG EVSSGSGETF SYPDFLRMML GKRSAILKMI LMYEEKAREK EKPTGPPAKK AISELPLEHH HHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | IFG Protein, IFI Protein, IFN gamma Protein, IFN, Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
16.9 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
11.7 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.6 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.3 kDa | |
E.Coli |
Unconjugated | |
95% | |
26.6 kDa | |
Human IFN-gamma R1, His Tag (orb257557) is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333). |
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