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Catalog Number | orb391998 |
---|---|
Category | Proteins |
Description | Recombinant human IL8 protein is produced by our E. coli expression system and the target gene encoding Ser28-Ser99 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IL8 |
Entrez | 3576 |
UniProt ID | P10145 |
Protein Sequence | SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | CXCL8; Interleukin-8; IL-8; C-X-C motif chemokine Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
8.4 kDa | |
E.Coli |
> 96% as determined by SDS-PAGE and HPLC. | |
8.9 kDa | |
E.Coli |
Unconjugated | |
90% | |
10.3 kDa | |
Human IL-8, His Tag (orb612149) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1). |
Unconjugated | |
95% | |
34.8 kDa | |
Human IL-8, Fc Tag (orb651990) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1). |
Unconjugated | |
90% | |
18.8 kDa | |
Human IL-17E, His Tag (orb1149282) is expressed from human 293 cells (HEK293). It contains AA Tyr 33 - Gly 177 (Accession # NP_073626.1). |
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