Human IL6 protein

• Catalog Number: orb80063
• Category: Proteins
• Description: Human IL6 protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Human IL6
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin-6 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 10,000,000 Units per mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
• Alternative names: Interleukin BSF 2 protein, B cell differentiation
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-6 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL6 protein