You have no items in your shopping cart.
Cart summary
Item 1 of 1
| Catalog Number | orb80063 |
|---|---|
| Category | Proteins |
| Description | Human IL6 protein |
| Target | Human IL6 |
| Conjugation | Unconjugated |
| Buffer/Preservatives | Lyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-6 as Reference Standard. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 10,000,000 Units per mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-6 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Alternative names | Interleukin BSF 2 protein, B cell differentiation Read more... |
| Note | For research use only |
Giampaolo Bosi 1, Paolo Merella 2, Emanuela Franchella 3, Bahram Sayyaf Dezfuli 4, Luisa Giari The Leydig organ of elasmobranchs: Shed light on an active and mysterious defense center with immunological characterization of its cells Tissue Cell, (2025)
SDS-PAGE analysis of Human IL6 protein
- Item 1 of 1
Human IL6 protein (Active) [orb358972]
> 96% as determined by SDS-PAGE and HPLC.
20.7 kDa
E.Coli
5 μg, 500 μg, 100 μg - Item 1 of 1
Human IL6 protein [orb594784]
Greater than 95% as determined by SDS-PAGE.
20.9 kDa
E.coli
1 mg, 500 μg, 50 μg, 10 μg - Item 1 of 3
Human IL6 Protein, mFc-His Tag [orb689393]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μg - Item 1 of 1
- Item 1 of 3
