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Catalog Number | orb391992 |
---|---|
Category | Proteins |
Description | Recombinant human IL6 protein is produced by our E.coli expression system and the target gene encoding Pro29-Met212 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 5.0 |
Purity | Greater than 98% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IL6 |
Entrez | 3569 |
UniProt ID | P05231 |
Protein Sequence | PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 5.0 |
Alternative names | IFNB2; Interleukin-6; IL-6; B-cell stimulatory fac Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
> 95% as determined by SDS-PAGE | |
20.8 kDa | |
Human IL-6, premium grade (orb257601) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000592.3). |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
Unconjugated | |
90% | |
23.6 kDa | |
Mouse IL-6, His Tag (orb750344) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1). |
Unconjugated | |
95% | |
22.9 kDa | |
Cynomolgus IL-6, His Tag (orb1152434) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Met 212 (Accession # P79341-1). |