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Catalog Number | orb358971 |
---|---|
Category | Proteins |
Description | Recombinant human IL5 active protein |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | > 98% as determined by SDS-PAGE and HPLC. |
MW | 13.3 kDa |
UniProt ID | P05113 |
Protein Sequence | M+IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES |
Protein Length | Full Length of Mature Protein |
Source | E.Coli |
Expression System | 20-134aa |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
Expression Region | 20-134aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 µm filtered 20mM PB, pH 7.5, 3 % trehalose, 0.1 % Tween-80 |
Alternative names | IL-5, Eosinophil differentiation factor, T-cell re Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
14.2 kDa | |
Mammalian cell |
Unconjugated | |
90% | |
15.2 kDa | |
Rabbit IL-5, His Tag (orb750340) is expressed from human 293 cells (HEK293). It contains AA Met 20 - Ser 134 (Accession # G1SL79-1). |
Unconjugated | |
90% | |
15.0 KDa | |
Mouse IL-5, His Tag (orb762422) is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1). |
Unconjugated | |
90% | |
15.0 kDa | |
Human IL-5, His Tag, premium grade (orb612148) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1). |
Unconjugated | |
90% | |
15.0 kDa | |
Cynomolgus IL-5, His Tag (orb864215) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # A0A2K5U1E7-1). |
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