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Catalog Number | orb80422 |
---|---|
Category | Proteins |
Description | Recombinant of Human IL4 protein (His Tag) |
Form/Appearance | Sterile Filtered clear solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS |
Source | Escherichia Coli |
Biological Activity | The ED50 for this effect is < 0.5ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles |
Buffer/Preservatives | Interleukin-4 His-Tag is supplied in 20mM Tris-HCl and10% glycerol. |
Alternative names | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymp Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 15.8 kDa after removal of the signal peptide. The apparent molecular mass of IL4-His is approximately 15-25 kDa due to glycosylation. | |
E.coli |
98.00% | |
17.8 kDa (predicted). Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE | |
Biotinylated Recombinant Human IL-4 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Ser153) of Human IL-4 fused with a His Tag and Avi tag at the C-terminal. |
≥90% as determined by SDS-PAGE | |
This protein contains the human IL4(His25-Ser153) was fused the C-terminal His Tag and expressed in E. coli. |
> 95% by Tris-Bis PAGE | |
Recombinant Human IL-4 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Ser153) of Human IL-4 fused with His Tag and Avi tag at the C-terminal. |