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Catalog Number | orb391967 |
---|---|
Category | Proteins |
Description | Recombinant human IL25 protein is produced by our mammalian expression system and the target gene encoding Tyr33-Gly177 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL25 |
Entrez | 64806 |
UniProt ID | Q9H293 |
Protein Sequence | YSHWPSCCPS KGQDTSEELL RWSTVPVPPL EPARPNRHPE SCRASEDGPL NSRAISPWRY ELDRDLNRLP QDLYHARCLC PHCVSLQTGS HMDPRGNSEL LYHNQTVFYR RPCHGEKGTH KGYCLERRLY RVSLACVCVR PRVMGVDHHH HHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Alternative names | IL17E; Interleukin-25; IL-25; Interleukin-17E; IL- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
18.8 kDa | |
Human IL-17E, His Tag (orb1149282) is expressed from human 293 cells (HEK293). It contains AA Tyr 33 - Gly 177 (Accession # NP_073626.1). |
Unconjugated | |
95% | |
56.7 kDa | |
Human IL-17 RB, Fc Tag (orb1496253) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Pro 292 (Accession # Q9NRM6-1 (EVI27) & Q9NRM6-1(IL17Rh1)). |
Human | |
Request Information | |
Request Information |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
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