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Catalog Number | orb391956 |
---|---|
Category | Proteins |
Description | Recombinant human IL20 protein is produced by our E. coli expression system and the target gene encoding Leu25-Glu176 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | IL20 |
Entrez | 50604 |
UniProt ID | Q9NYY1 |
Protein Sequence | MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | ZCYTO10; Interleukin-20; IL-20; Cytokine Zcyto10 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
17.6 kDa | |
E.Coli |
Unconjugated | |
95% | |
51.7 kDa | |
Human IL-20 R alpha, Fc Tag (orb1146994) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Lys 250 (Accession # Q9UHF4-1). |
Unconjugated | |
95% | |
50.9 kDa | |
Human IL-22 R alpha 1, Fc Tag (orb1289953) is expressed from human 293 cells (HEK293). It contains AA His 16 - Thr 228 (Accession # Q8N6P7-1). |
Unconjugated | |
95% | |
44.6 kDa | |
Human IL-17B, Fc Tag (orb1496165) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Phe 180 (Accession # Q9UHF5-1). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.7 kDa after removal of the signal peptide. The apparent molecular mass of IL20-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
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